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(A) Fold-change in PGE 2 release in FAK+/+ and FAK−/− osteoblasts following 2 hours of oscillatory flow and a 4 hour post-flow incubation. FAK−/− osteoblasts exhibited a significantly lower fold-change in PGE 2 release in response to fluid flow compared to FAK+/+ osteoblasts. (B) Fold-change in PGE2 release following fluid flow in mutant osteoblasts transiently re-expressing wild-type focal adhesion kinase and <t>phosphospecific</t> site mutations. Note that differences in absolute fold-changes in PGE2 release following flow in Panel A and Panel B represent variations in parallel assays.
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(A) Fold-change in PGE 2 release in FAK+/+ and FAK−/− osteoblasts following 2 hours of oscillatory flow and a 4 hour post-flow incubation. FAK−/− osteoblasts exhibited a significantly lower fold-change in PGE 2 release in response to fluid flow compared to FAK+/+ osteoblasts. (B) Fold-change in PGE2 release following fluid flow in mutant osteoblasts transiently re-expressing wild-type focal adhesion kinase and <t>phosphospecific</t> site mutations. Note that differences in absolute fold-changes in PGE2 release following flow in Panel A and Panel B represent variations in parallel assays.
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(A) Fold-change in PGE 2 release in FAK+/+ and FAK−/− osteoblasts following 2 hours of oscillatory flow and a 4 hour post-flow incubation. FAK−/− osteoblasts exhibited a significantly lower fold-change in PGE 2 release in response to fluid flow compared to FAK+/+ osteoblasts. (B) Fold-change in PGE2 release following fluid flow in mutant osteoblasts transiently re-expressing wild-type focal adhesion kinase and <t>phosphospecific</t> site mutations. Note that differences in absolute fold-changes in PGE2 release following flow in Panel A and Panel B represent variations in parallel assays.
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(A) Fold-change in PGE 2 release in FAK+/+ and FAK−/− osteoblasts following 2 hours of oscillatory flow and a 4 hour post-flow incubation. FAK−/− osteoblasts exhibited a significantly lower fold-change in PGE 2 release in response to fluid flow compared to FAK+/+ osteoblasts. (B) Fold-change in PGE2 release following fluid flow in mutant osteoblasts transiently re-expressing wild-type focal adhesion kinase and <t>phosphospecific</t> site mutations. Note that differences in absolute fold-changes in PGE2 release following flow in Panel A and Panel B represent variations in parallel assays.
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(A) Fold-change in PGE 2 release in FAK+/+ and FAK−/− osteoblasts following 2 hours of oscillatory flow and a 4 hour post-flow incubation. FAK−/− osteoblasts exhibited a significantly lower fold-change in PGE 2 release in response to fluid flow compared to FAK+/+ osteoblasts. (B) Fold-change in PGE2 release following fluid flow in mutant osteoblasts transiently re-expressing wild-type focal adhesion kinase and <t>phosphospecific</t> site mutations. Note that differences in absolute fold-changes in PGE2 release following flow in Panel A and Panel B represent variations in parallel assays.
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(A) Fold-change in PGE 2 release in FAK+/+ and FAK−/− osteoblasts following 2 hours of oscillatory flow and a 4 hour post-flow incubation. FAK−/− osteoblasts exhibited a significantly lower fold-change in PGE 2 release in response to fluid flow compared to FAK+/+ osteoblasts. (B) Fold-change in PGE2 release following fluid flow in mutant osteoblasts transiently re-expressing wild-type focal adhesion kinase and phosphospecific site mutations. Note that differences in absolute fold-changes in PGE2 release following flow in Panel A and Panel B represent variations in parallel assays.

Journal: PLoS ONE

Article Title: Focal Adhesion Kinase Plays a Role in Osteoblast Mechanotransduction In Vitro but Does Not Affect Load-Induced Bone Formation In Vivo

doi: 10.1371/journal.pone.0043291

Figure Lengend Snippet: (A) Fold-change in PGE 2 release in FAK+/+ and FAK−/− osteoblasts following 2 hours of oscillatory flow and a 4 hour post-flow incubation. FAK−/− osteoblasts exhibited a significantly lower fold-change in PGE 2 release in response to fluid flow compared to FAK+/+ osteoblasts. (B) Fold-change in PGE2 release following fluid flow in mutant osteoblasts transiently re-expressing wild-type focal adhesion kinase and phosphospecific site mutations. Note that differences in absolute fold-changes in PGE2 release following flow in Panel A and Panel B represent variations in parallel assays.

Article Snippet: Primary antibodies used were anti-mouse monoclonal FAK antibody (clone 4.47) (Millipore), rabbit polyclonal antibody against phosphospecific FAK Tyr-397 (Santa Cruz Biotechnology), rabbit polyclonal antibody against phosphospecific p-proline-rich tyrosine kinase 2 (Pyk2)-Tyr 402 conjugated to Alexa Fluor 488 (Invitrogen), chicken antibody against paxillin (clone 349) (BD Biosciences), monoclonal anti-vinculin (clone hVIN-1) (Sigma) and actin mouse monoclonal antibody (clone AC-40) (Abcam).

Techniques: Incubation, Mutagenesis, Expressing